Journal: Frontiers in Molecular Biosciences

Article Title: Deciphering the circRNA-Mediated ceRNA regulatory network in dendritic cells during H37Ra and BCG infection

doi: 10.3389/fmolb.2026.1764518

Figure Lengend Snippet: Flow cytometric analysis of CD1a and CD83 expression on the surface of DCs at different time points. (A) Expression of CDla on Day 1 of Culture; (B) Expression of CDla on Day 8 of Culture; (C) Statistical Analysis Results of CDla on Day 1 and Day 8 of Culture; (D) Expression of CD83 on Day 1 of Culture; (E) Expression of CD83 on Day 8 of Culture; (F) Statistical Analysis Results of CD83 on Day 1 and Day 8 of Culture. It is evident that the maturation rate of DCs induced by GM-CSF and IL-4 has been significantly enhanced, thereby facilitating sample preparation for subsequent experiments.Statistical significance is indicated in the figure (*p < 0.05, **P < 0.01, ***P < 0.001).

Article Snippet: Subsequently, 100 μL of the cell suspension was separately incubated with 5 μL of APC Anti-Human CD1a Antibody and PE Anti-Human CD83 Antibody 5 μL of antibodies (Elabscience Biotechnology Co., Ltd., Wuhan, China).The samples were mixed thoroughly and incubated for 30 min at room temperature in the dark.

Techniques: Expressing, Sample Prep

Journal: Virologica Sinica

Article Title: Host factor Naf1 restricts HIV-1 infection of myeloid cells and compromises the capacity of dendritic cell to prime CD4 + T cell

doi: 10.1016/j.virs.2025.03.007

Figure Lengend Snippet: Naf1 knockdown increases NF-κB activation and cytokines expression. A Naf1 knockdown in MDDCs during the treatment with pseudo-typed HIV-luc/JRFL (10 ​ng p24 gag amount virus). B Expressions of cytokines and chemokines. MDDCs (5 ​× ​10 5 ​cells) with or without Naf1 knockdown were incubated in presence or absence of LPS (10 ​ng/mL) for 6 ​h, and cell total mRNAs were extracted, and the cytokines and chemokines were measured with real-time (RT-) PCR. Data are presented as mean ​± ​SD from three representative experiments. ∗∗∗ P ​< ​0.001 was considered as significant difference in unpaired t -test. C, D MDDCs (5 ​× ​10 5 ​cells) with or without Naf1 knockdown were used to detect the surface expressions of CD83, CD86 and HLA-DR with flow cytometry, and results from three donors were summarized ( D ).

Article Snippet: Monoclonal anti-human antibodies against the following molecules were used in immunostaining: PE-CD83 (HB15e, eBioscience, San Diego, CA, USA); PE-CD86 (IT2.2, eBioscience); APC-cy7-HLA-DR (LN3, eBioscience); PE-ICAM-1 (HA58, eBioscience); PerCP-cy5.5-CD3 (OKT3, eBioscience) and PE-CD69 (FN50, eBioscience).

Techniques: Knockdown, Activation Assay, Expressing, Virus, Incubation, Quantitative RT-PCR, Flow Cytometry